Had the MOAP (mother-of-all-punctures) last night. Took me three attempts to fix it and ended up using a spare tube. Lesson learned there. Always use the new tube first! It looks like the latex sealant I was using in my tubes had not dried out but had lost the ability to dry and plug holes. Could have been due to the temperature which was near freezing. Can't really complain though. I have been running the JRA wheel milk in my tubes since September last year and this is the first time I have had to attend to a puncture since then.